Outer Membrane Protein Insertion by the β-barrel Assembly Machine
نویسندگان
چکیده
منابع مشابه
β-Barrel membrane protein assembly by the Bam complex.
β-barrel membrane proteins perform important functions in the outer membranes (OMs) of Gram-negative bacteria and of the mitochondria and chloroplasts of eukaryotes. The protein complexes that assemble these proteins in their respective membranes have been identified and shown to contain a component that has been conserved from bacteria to humans. β-barrel proteins are handled differently from ...
متن کاملStructure and function of BamE within the outer membrane and the β-barrel assembly machine.
Insertion of folded proteins into the outer membrane of Gram-negative bacteria is mediated by the essential β-barrel assembly machine (Bam). Here, we report the native structure and mechanism of a core component of this complex, BamE, and show that it is exclusively monomeric in its native environment of the periplasm, but is able to adopt a distinct dimeric conformation in the cytoplasm. BamE ...
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Outer membrane β-barrel proteins spontaneously fold into lipid bilayers with rates of folding that are strongly influenced by the physical properties of the membrane. We show that folding is accelerated when the bilayer is at the phase transition temperature, because of the coexistence of lipid phase domains and the high degree of defects present at domain boundaries. These results are consiste...
متن کاملThe -Barrel Outer Membrane Protein Assembly Complex of Neisseria meningitidis
The evolutionarily conserved protein Omp85 is required for outer membrane protein (OMP) assembly in gram-negative bacteria and in mitochondria. Its Escherichia coli homolog, designated BamA, functions with four accessory lipoproteins, BamB, BamC, BamD, and BamE, together forming the -barrel assembly machinery (Bam). Here, we addressed the composition of this machinery and the function of its co...
متن کاملTwo novel proteins in the mitochondrial outer membrane mediate β-barrel protein assembly
Mitochondrial outer and inner membranes contain translocators that achieve protein translocation across and/or insertion into the membranes. Recent evidence has shown that mitochondrial beta-barrel protein assembly in the outer membrane requires specific translocator proteins in addition to the components of the general translocator complex in the outer membrane, the TOM40 complex. Here we repo...
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ژورنال
عنوان ژورنال: EcoSal Plus
سال: 2019
ISSN: 2324-6200
DOI: 10.1128/ecosalplus.esp-0035-2018